Molecular docking and inhibition profiles of some antibiotics on lactoperoxidase enzyme purified from bovine milk
Yazarlar (10)
Ramazan Kalın Erzurum Teknik Üniversitesi, Türkiye
Zeynep Köksal İstanbul Medeniyet Üniversitesi, Türkiye
Songul Bayrak
Serpil Gerni
Isil Nihan Ozyurek
Dr. Öğr. Üyesi Hande USANMAZ Sinop Üniversitesi, Türkiye
Muhammet Karaman Kilis 7 Aralık Üniversitesi, Türkiye
Ali Atasever Atatürk Üniversitesi, Türkiye
Prof. Dr. Hasan Özdemir Atatürk Üniversitesi, Türkiye
İlhami Gülçin Atatürk Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (Q1) | ||
| Dergi ISSN | 0739-1102 Dergi Bilgileri (2022) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2022 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 28-08-2020 |
| Cilt / Sayı / Sayfa | 40 / 1 / 401–410 | DOI | 10.1080/07391102.2020.1814416 |
| Makale Linki | http://dx.doi.org/10.1080/07391102.2020.1814416 | ||
| UAK Araştırma Alanları |
Biyokimya
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| Özet |
| Antibiotics are generally used for human and veterinary applications to preserve and to control microbial diseases. Milk has a biologically significant enzyme known as lactoperoxidase (LPO) that is a member of peroxidase family. In metabolism, LPO has ability to catalyze the transformation of thiocyanate (SCN-) to hypothiocyanite (OSCN-) that is an antibacterial agent and the reaction occurs with hydrogen peroxide. In this work, LPO inhibition effects of some antibiotics including cefazolin, oxytetracycline, flunixin meglumine, cefuroxime, tylosin, vancomycin, chloramphenicol and lincomycin were tested. Among the antibiotics cefazolin was indicated the strongest inhibitory efficacy. The half maximal inhibitory concentration (IC50) and the inhibition constant (Ki) values of cefazolin were found as 8.19 and 34.66 µM, respectively. It was shown competitive inhibition. 5-Methyl-1,3,4-thiadiazol-2-yl moiety activity plays a … |
| Anahtar Kelimeler |
| Lactoperoxidase | enzyme purification | antibiotics | kinetics | enzyme inhibition |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 13 |
| Google Scholar | 16 |