Inhibition effects of some phenolic and dimeric phenolic compounds on bovine lactoperoxidase LPO enzyme
Yazarlar (3)
Zeynep Köksal İstanbul Medeniyet Üniversitesi, Türkiye
Dr. Öğr. Üyesi Hande USANMAZ Sinop Üniversitesi, Türkiye
Hasan Özdemir
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | International Journal of Academic Research | ||
| Dergi ISSN | 2075-4124 | ||
| Dergi Tarandığı Indeksler | AHCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2014 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 30-03-2014 |
| Cilt / Sayı / Sayfa | 6 / 2 / – | DOI | 10.7813/2075-4124.2014/6-2 |
| Makale Linki | http://ijar.lit.az/en.php?go=march2014 | ||
| UAK Araştırma Alanları |
Biyokimya
|
||
| Özet |
| Antioxidants and antioxidative enzymes transform the toxic effects of reactive oxygen species to harmless substances. Lactoperoxidase (LPO), the most crucial enzyme in milk, is a member of Peroxidases. In this study, some important drug substances, investigated the in vitro inhibitory effects on LPO enzyme. Bovine LPO enzyme was purified with using Amberlite CG-50 H+ resin and affinity chromatography. LPO was purified 406-fold from the synthesized affinity chromatography with a yield 74.24% and a specific activity of 29.69 EU/mg protein. The enzyme activity was measured using by ABTS substrate (pH 6.0). Soluble in organic solvents 2,6-Dimethylphenol, 2,6-di-t-butylphenol, di(2,6-dimethylphenol), di(2,6-diisopropylphenol), and di(2,6-di-t-butylphenol) were used as the active ingredient of a pharmaceutical agent. All these phenols were investigated here showed strong LPO inhibitory effects and competitive … |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 12 |