Silver nanoparticles: biosynthesis and cytotoxic performance against breast cancer MCF-7 and MDA-MB-231 cell lines
Yazarlar (6)
Soodabeh Darvish Ayatollah Taleghani Educational Hospital
Mohammad Saeed Kahrizi
Alborz University Of Medical Sciences
Alborz University Of Medical Sciences
Doç. Dr. Gülüzar ÖZBOLAT Sinop Üniversitesi, Türkiye
Fatemeh Khaleghi
Shahid Sadoughi University Of Medical Sciences
Shahid Sadoughi University Of Medical Sciences
Zohreh Mortezania
Sbums School Of Medicine
Sbums School Of Medicine
Delaram Sakhaei
Islamic Azad University, Sari Branch
Islamic Azad University, Sari Branch
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Nanomedicine Research Journal | ||
| Dergi ISSN | 2476-3489 Dergi Bilgileri (2022) | ||
| Dergi Tarandığı Indeksler | SCOPUS, Biological Abstracts, BIOSIS Previews | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2022 |
| Cilt / Sayı / Sayfa | 7 / 1 / 83–92 | DOI | 10.22034/nmrj.2022.01.008 |
| Makale Linki | http://www.nanomedicine-rj.com/article_251673.html | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| Objective(s): A study was performed on the possibility of synthesizing silver nanoparticles (Ag-NPs) from Ducrosia Anethifolia Aqueous Extract for treating breast cancer MCF-7 and MDA-MB-231 cell lines.Methods The application of Ducrosia Anethifolia Aqueous Extract caused a reduction in silver ions and facilitated the production of silver nanoparticles. Different analyzing procedures were exerted to evaluate the qualities of synthesized NPs by Ducrosia Anethifolia Aqueous Extract including UV-Vis spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). In the following, subsequent to assessing the viability of MCF-7 and MDA-MB-231 cell lines, their exposure to AgNPs was evaluated by the usage of MTT assay. Moreover, the Bax, Bad, Bcl-2, and c-FLIP expression levels of treated cells were examined by real-time PCR analysis.Results The observance of an absorption peak at 460 nm through the obtained data from a spectrophotometer affirmed the production of silver NPs. The images of electron microscope displayed the spherical shape of NPs in an average size of around 9.41 nm. Moreover, this product caused a robust reduction in the viability of both MCF-7 and MDA-MB-231 cell lines that was mainly pointed out by the improved expressions of Bax and Bad gene, while minimizing the gene expressions of Bcl-2 and c-FLIP.Conclusions This experiment approved the applicability of Ducrosia Anethifolia Aqueous Extract for performing the green synthesis of NPs. In addition, the synthesized NPs exhibited anti-tumor effects on the applied breast cancer cell lines through the up-regulation of pro-apoptotic … |
| Anahtar Kelimeler |
| biosynthesis | breast cancer | Ducrosia Anethifolia | Silver nanoparticle | viability |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 24 |
| Google Scholar | 27 |