Fast heme release from inactive proteins
Yazarlar (3)
| Makale Türü | Özgün Makale (Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale) | ||
| Dergi Adı | Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology | ||
| Makale Dili | – | Basım Tarihi | 09-1991 |
| Cilt / Sayı / Sayfa | 1079 / 3 / 330–334 | DOI | – |
| Makale Linki | https://www.sciencedirect.com/science/article/pii/016748389190077D | ||
| UAK Araştırma Alanları |
Dedektör Teknolojisi
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| Özet |
| Kinetics for the release of the prosthetic group from hemoproteins is presented. Heme-protein separation is a biphasic reaction, where an initial phase is significantly faster than the dominant, slow phase. A previous communication concluded that the slow phase represents the active protein. This communication presents data for porphyrin release which shows that the initial fast phase represents an inactive form of the protein. Moreover, we suggest that the fast to slow phase ratio is a sensitive monitor of sample quality for many hemoproteins and that an extrapolation of the slow phase absorbance leads to new estimates for the true physical parameters of unperturbed proteins. |
| Anahtar Kelimeler |
