Molecular characterization of demersal marine fish species Pseudorhombus arsius, Psettods erumei, and Cynoglossus cynoglossus from Sindh coasts of Pakistan through DNA barcodes
Yazarlar (4)
| Makale Türü | Özgün Makale (Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale) | ||
| Dergi Adı | J. Mater. Environ. Sci., 14 (2), 210 | ||
| Makale Dili | – | Basım Tarihi | 01-2023 |
| Cilt / Sayı / Sayfa | 223 / 0 / – | DOI | – |
| Makale Linki | https://www.researchgate.net/profile/Quratulan-Ahmed/publication/368720129_Molecular_characterization_of_demersal_marine_fish_species_Pseudorhombus_arsius_Hamilton_1822_Psettods_erumei_Bloch_Schneider_1801_and_Cynoglossus_cynoglossus_Hamilton_1822_fr | ||
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Hidrobiyoloji
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| Özet |
| External morphological characteristics have traditionally been used to identify fish species. However, it can often be challenging to distinguish fishes by their morphological characteristics, particularly given their many developmental stages. Molecular-based identification creates a quick, precise, and affordable system for identifying species through DNA barcoding. To establish sequence profiles of known species versus sequences of unknowns that can be matched and subsequently identified, it uses short, conventional gene targets. Pseudorhombus arsius, Psettods erumei and Cynoglossus cynoglossus specimens were collected from two stations I: Kharochan,(67◦ 34’42’N, 24◦ 04’31E); II: Jhangisir (67◦ 37’34’N, 24◦ 11’18E); Sindh coast, Pakistan, during July 2020 to March 2021. The sequence of P. arsius DNA barcoding indicated (597 bases in 7258 scans); base spacing: 15.571008;(G: 128 A: 159 C: 212 T: 201); C. cynoglossus DNA barcoding indicated (681 bp in 7258 scans); Base spacing: 15.571008;(G: 228 A: 169 C: 112 T: 171); and P. erumei DNA barcoding indicated (680 bp in 7258 scans); Base spacing: 15.571008,(G: 182 A: 129 C: 242 T: 127). This is the first study that provides information on morphological and molecular-based identification from Pakistan’s coasts of the Arabian Sea. DNA-based identification techniques provide a significant analytical complement or possibly a substitute for inventory for future biologists. |
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