Predictive value of KRAS/NRAS, IL-4 VNTR and HPV alterations in metastases of colorectal cancer
Yazarlar (6)
Ozen Filiz
Goztepe Prof. Dr. Suleyman Yalcin City Hospital, Türkiye
Goztepe Prof. Dr. Suleyman Yalcin City Hospital, Türkiye
Doç. Dr. Zeynep YEĞİN Sinop Üniversitesi, Türkiye
Avsar Cumhur
Sinop Üniversitesi, Türkiye
Sinop Üniversitesi, Türkiye
Zenginkinet Tulay
Goztepe Prof. Dr. Suleyman Yalcin City Hospital, Türkiye
Goztepe Prof. Dr. Suleyman Yalcin City Hospital, Türkiye
Koc Haydar
Çankiri Karatekin Üniversitesi, Türkiye
Çankiri Karatekin Üniversitesi, Türkiye
Ulasoglu Celal
Istanbul Okan University, Türkiye
Istanbul Okan University, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Bratislava Medical Journal (Q2) | ||
| Dergi ISSN | 0006-9248 Dergi Bilgileri (2022) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 10-2022 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 23-07-2022 |
| Cilt / Sayı / Sayfa | 123 / 10 / 758–768 | DOI | 10.4149/BLL_2022_122 |
| Makale Linki | http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7762&category_id=164&option=com_virtuemart&Itemid=1 | ||
| UAK Araştırma Alanları |
Tıbbi Biyoloji
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| Özet |
| PURPOSE The main aim of the study was to evaluate the potential roles of KRAS/NRAS proto-oncogenes, IL-4 VNTR variants and HPV prevalence in colorectal cancer metastasis. As the second aim, the interactions of the analyzed genes and viral sequences with both clinicopathological variables and each other were targeted. METHODS DNA was extracted using AmoyDx FFPE DNA Extraction kit from paraffin-embedded colorectal tumor tissue samples (n= 60). NRAS/KRAS mutational profiles were determined with real-time polymerase chain reaction using AmoyDx KRAS/NRAS Mutation Detection Kit. Genotyping of IL-4 VNTR was made with PCR. HPV detection was analyzed by PCR with both GP5+/GP6+ consensus primers and type-specific primers for HPV-16 and HPV-18. SPSS v22 (IBM) statistics software was used for all statistical analyses. RESULTS From the demographical/clinicopathological parameters, age and biopsy specimens revealed an association with metastasis. KRAS mutation rate was as high as 65% in the patients and the most prevalent mutation type was G12D. Metastasis risk was 3.19-fold increased in KRAS-mutated patients compared to KRAS-negative ones. IL-4 VNTR genotypes/alleles were not associated with metastasis in our analysis. The frequency of HPVs in our colorectal cancer cohort was 36.7%, but HPV positivity was not found to be associated with metastasis. A significant association was found between HPV and NRAS mutations; NRAS wild-type status acted as a protective factor by 7.5-fold for HPV negativity. CONCLUSION Our study comprehensively and concomitantly evaluated several potential … |
| Anahtar Kelimeler |
| Colorectal cancer | Human papillomavirus | Il-4 | Kras | Metastasis | Nras |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 2 |
| Google Scholar | 4 |