Characterization of Environmental Bacillus Isolates by Protein Fingerprinting and Random Amplified Polymorphic DNA Profiles
Yazarlar (4)
İsmet Berber
Sinop Üniversitesi, Türkiye
Sinop Üniversitesi, Türkiye
Doç. Dr. Cumhur AVŞAR Sinop Üniversitesi, Türkiye
Doç. Dr. Zeynep YEĞİN Sinop Üniversitesi, Türkiye
Seyhan Civek
Sinop Üniversitesi, Türkiye
Sinop Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Proceedings of the National Academy of Sciences India Section B Biological Sciences | ||
| Dergi ISSN | 0369-8211 Dergi Bilgileri (2017) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2017 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 23-01-2016 |
| Cilt / Sayı / Sayfa | 87 / 4 / 1247–1259 | DOI | 10.1007/s40011-015-0700-0 |
| Makale Linki | http://dx.doi.org/10.1007/s40011-015-0700-0 | ||
| UAK Araştırma Alanları |
Tıbbi Biyoloji
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| Özet |
| The aim of the present study was to investigate antibiotic resistance, plasmid, random amplified polymorphic DNA (RAPD) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profiles of the Bacillus spp. isolated from soil of Sinop environs, Turkey. A total of fifty-seven isolates were identified as Bacillus spp. based on morphological and physiological properties. All isolates were found resistant to bacitracin. Susceptibility patterns of the isolates to other tested antibiotics varied from 100 to 31.5 %. The plasmid analysis results indicated that isolates harbored 2 to 7 plasmids. The numerical analysis of whole cell and extracellular protein profiles of the isolates displayed similarity levels between 78–100 and 67–100 % respectively reflecting the less discriminative power of SDS-PAGE at subspecies level. However, RAPD analysis of genomic DNA using four different universal primers revealed 100 … |
| Anahtar Kelimeler |
| Antibiotic resistance | Bacillus | Molecular characterization | Protein fingerprinting | Random amplified polymorphic DNA |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 1 |
| Google Scholar | 1 |