Enhanced kinetic performance and stability of catalase immobilized on epoxy-functionalized kaolinite
Yazarlar (6)
Prof. Dr. Kadir Erol Hitit University, Türkiye
Doç. Dr. Aysel VEYİSOĞLU Sinop Üniversitesi, Türkiye
Doç. Dr. Demet Tatar Hitit University, Türkiye
Öğr. Gör. Buket Bulut Kocabaş Istanbul Arel Üniversitesi, Türkiye
Doç. Dr. İhan Alacabey Mardin Artuklu University, Türkiye
Prof. Dr. Ebru Gökmeşe Hitit University, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Scientific Reports (Q1) | ||
| Dergi ISSN | 2045-2322 Dergi Bilgileri (2026) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2026 |
| Cilt / Sayı / Sayfa | 16 / 1 / 8196–0 | DOI | 10.1038/s41598-026-38910-z |
| Makale Linki | https://doi.org/10.1038/s41598-026-38910-z | ||
| UAK Araştırma Alanları |
Mikrobiyoloji
Bakteriyoloji
Moleküler Biyoloji
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| Özet |
| The immobilization of catalase onto stable, reusable supports is crucial for efficient peroxide-based biocatalytic applications. In this study, catalase was immobilized for the first time onto epoxy-functionalized kaolinite particles prepared via surface silanization with (3-glycidyloxypropyl)trimethoxysilane. Structural and surface characterizations confirmed successful organosilane grafting while preserving the layered kaolinite framework. The modified support exhibited rapid enzyme uptake and a high immobilization capacity of approximately 300 mg g−1. Kinetic analysis showed a substantial decrease in Km from 57.3 mM (free catalase) to 21.6 mM after immobilization, indicating enhanced substrate affinity. In contrast, Vmax decreased due to diffusion limitations typical of heterogeneous systems. Despite this, catalytic efficiency increased nearly 1.8-fold. Moreover, immobilized catalase demonstrated significantly … |
| Anahtar Kelimeler |
| Catalase | Immobilization | Kaolinite | Kinetics | Silanization | Stability |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 3 |
| Scopus | 1 |
| Google Scholar | 3 |