The Effects of Cannabinoid Agonist, Heat Shock Protein 90 and Nitric Oxide Synthase Inhibitors on Increasing IL-13 and IL-31 Levels in Chronic Pruritus
Yazarlar (4)
Dr. Öğr. Üyesi Zeynep Gizem Todurga Seven Haliç Üniversitesi, Türkiye
Prof. Dr. Ayşe GÜNDOĞDU Sinop Üniversitesi, Türkiye
Rumeysa Ozyurt
Kütahya Sağlık Bilimleri Üniversitesi, Türkiye
Kütahya Sağlık Bilimleri Üniversitesi, Türkiye
Prof. Dr. Sibel Özyazgan İstanbul University-Cerrahpaşa Cerrahpaşa Faculty of Medicine, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Immunological Investigations (Q3) | ||
| Dergi ISSN | 0882-0139 Dergi Bilgileri (2022) | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2022 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 08-06-2022 |
| Cilt / Sayı / Sayfa | 51 / 7 / 1938–1949 | DOI | 10.1080/08820139.2022.2083973 |
| Makale Linki | https://www.tandfonline.com/doi/full/10.1080/08820139.2022.2083973 | ||
| UAK Araştırma Alanları |
Ziraat, Orman ve Su Ürünleri
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| Özet |
| Background: Heat shock protein 90 (Hsp90) inhibitor and cannabinoid agonists ameliorate dry skin-induced chronic itch. We have recently reported that cannabinoids, hsp90 and nitric oxide (NO) are involved in dry skin-induced itch. Here, we investigated the contribution of the Th2 cell signaling pathway to the antipruritic effect of the hsp90 inhibitor 17-Alilamino-17-demethoxygeldanamycin (17-AAG), nitric oxide synthase (NOS) inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) and cannabinoid agonist WIN 55,212–2 on a dry skin-induced scratch. Methods: Dry skin-induced chronic itching was created by topical application of AEW (acetone/diethyl ether/water). WIN 55,212–2 (1 mg/kg, i.p.), L-NAME (1 mg/kg, i.p.) and increasing doses of 17-AAG (1, 3 and 5 mg/kg,i.p.) were administered to Balb/c mice (for each group, n = 6). After these applications, skin tissues were taken from the nape region of all of the mice. Gene and protein expressions of IL-13 and IL-31 were evaluated in skin tissues by RT-PCR and immunohistochemistry, respectively. Results: IL-13 and IL-31 mRNA expressions and immune positive cell counts were increased in the AEW applied groups. WIN 55,212–2 reduced both of the increased cytokines levels, while L-NAME decreased only the IL-13. 17-AAG dose-dependently reduced the increased cytokine levels. IL-13 and IL-31 levels significantly decreased following the co-administration of these agents. Conclusion: These results show that increased levels of IL-13 and IL-31 are associated with pruritus. Hsp90 inhibition and cannabinoid system activation may induce antipruritic effects through down-regulation of these cytokines. |
| Anahtar Kelimeler |
| Cannabinoid | chronic itch | hsp90 | IL-13 | IL-31 |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 6 |