Gum Arabic Modulates Redox–Ionic Microenvironments via Rheology and Kinetics to Induce Selective Cytotoxicity in Colorectal Cancer Cells
Yazarlar (12)
Emre Cebeci
Türkiye
Türkiye
Arş. Gör. Büşra Yüksel Türkiye
Reyhan Aliusta
Şahin Yılmaz
Türkiye
Türkiye
Dr. Öğr. Üyesi Ertuğrul Osman BURSALIOĞLU Sinop Üniversitesi, Türkiye
Mustafa Eray Bozyel
Türkiye
Türkiye
Dr. Öğr. Üyesi Şaban Kalay Türkiye
Dr. Öğr. Üyesi Ahmet Arif Kurt Türkiye
Prof. Dr. Fikrettin Şahin Türkiye
Doç. Dr. İsmail Aslan Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | GELS (Q1) | ||
| Dergi ISSN | 2310-2861 Dergi Bilgileri (2026) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2026 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 03-02-2026 |
| Cilt / Sayı / Sayfa | 12 / 2 / 1–18 | DOI | 10.3390/gels12020139 |
| Makale Linki | https://www.mdpi.com/2310-2861/12/2/139 | ||
| UAK Araştırma Alanları |
Biyoteknoloji
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| Özet |
| Background Gum Arabic (GA) is a natural polysaccharide widely recognized for its antioxidant and anti-inflammatory properties; however, its functional behavior as a biopolymeric gel and the mechanisms underlying its selective effects on cancer-related redox microenvironments remain insufficiently characterized. It is imperative to note that the interaction between its physicochemical properties and its biological activity in colorectal cancer remains to be fully clarified. Methods This study aimed to evaluate the antineoplastic potential of GA in human colorectal cancer (CRC) cell lines (HT-29 and HCT-116) compared to normal fibroblasts (MRC-5) using the MTS assay. Oxidative stress-related molecular responses were assessed by quantitative PCR analysis of GPX4, GSTA2, CAT, NFKB, and SOD1 expression. In parallel, extracellular concentrations of key metal ions (Fe2+, Zn2+, Mn2+, Mg2+, Cu2+, and Al3+) were quantified following GA exposure. To establish its functional gel characteristics, rheological measurements were performed to assess viscosity and shear-dependent behavior, and USP-compliant in vitro kinetic studies were conducted to evaluate time-dependent release properties. Results GA induced dose-dependent cytotoxicity in HT-29 and HCT-116 colorectal cancer cells, while MRC-5 fibroblasts exhibited comparatively higher viability across the tested concentration range, indicating reduced sensitivity in normal cells. Rheological analysis revealed concentration- and ion-dependent viscoelastic behavior, identifying a 10% (w/w) GA formulation as optimal due to its balanced low-shear viscosity and controlled shear-thinning … |
| Anahtar Kelimeler |
| Gum Arabic | functional gels | rheology | kinetic behavior | redox modulation | colorectal cancer |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 1 |
| Google Scholar | 2 |