Production of androgenetic Nile tilapia Oreochromis niloticus L optimization of heat shock duration and application time to induce diploidy
Yazarlar (4)
Prof. Dr. Sedat KARAYÜCEL Sinop Üniversitesi, Türkiye
Prof. Dr. İsmihan KARAYÜCEL Sinop Üniversitesi, Türkiye
David Penman
University Of Stirling, İngiltere
University Of Stirling, İngiltere
Brendan Mcandrew
University Of Stirling, İngiltere
University Of Stirling, İngiltere
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Israeli Journal of Aquaculture Bamidgeh (Q4) | ||
| Dergi ISSN | 0792-156X Dergi Bilgileri (2002) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2002 |
| Cilt / Sayı / Sayfa | 54 / 4 / 145–156 | DOI | – |
| UAK Araştırma Alanları |
Su Ürünleri Yetiştiriciliği
|
||
| Özet |
| Diploid androgenetic Nile tilapia, Oreochromis niloticus, were produced using 5 min UV irradia-tion at 150 μW/cm and heat shock at 42.5 oC for 3: 30 min applied 25 min after fertilization. The survival rates to morula, pigmentation, hatching and yolk sac resorption stages were 42.84±4.73%, 10.69±2.24%, 2.03±0.60% and 0.07±0.07%(relative to controls), respectively. A significant female, but not male, effect on the survival was confirmed. Multilocus DNA finger-printing produced by hypervariable 33.15 DNA probe verified the all-paternal inheritance in the androgens. The sex ratio of the androgenetic tilapia was not significantly different from the expected ratio of 1: 0 (male: female), indicating that O. niloticus has a monofactorial sex-deter-mining mechanism with female homogamety and male heterogamety. This study confirmed that the YY genotype is viable and fertile in O. niloticus. |
| Anahtar Kelimeler |
| Androgenesis | Diploidy | Tilapia |
