Investigation of oxidative stress status in cumulus cells in patıents with in vitro fertilization
Yazarlar (6)
Dr. Öğr. Üyesi Rabia TURAL Sinop Üniversitesi, Türkiye
Prof. Dr. Cengiz Karakaya Gazi Üniversitesi, Türkiye
Prof. Dr. Mehmet Erdem Gazi Üniversitesi, Türkiye
Zeynep Aykol
Ankara Private Ivf Center, Türkiye
Ankara Private Ivf Center, Türkiye
Recep Onur Karabacak Gazi University, Faculty Of Medicine, Türkiye
Prof. Dr. Mustafa Kavutçu Gazi University, Faculty Of Medicine, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Turkish Journal of Medical Sciences (Q2) | ||
| Dergi ISSN | 1300-0144 Dergi Bilgileri (2021) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2021 |
| Cilt / Sayı / Sayfa | 51 / 4 / 1969–1975 | DOI | 10.3906/sag-2104-188 |
| Makale Linki | http://dx.doi.org/10.3906/sag-2104-188 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| Background/aim The negative impact of oxidative stress on oocytes obtained from in vitro fertilization (IVF) patients is a challenge for the optimization of live birth rates. In this study, it is aimed to investigate whether oxidant/antioxidant parameters have a predictive value in terms of determining the count and quality of oocytes. Materials and methods Catalase (CAT), glutathione-S-transferase (GST), arylesterase (ARE) enzyme activities, and malondialdehyde (MDA) levels were analysed in cumulus cells of poor responder (n= 28, oocyte count≤ 4), normo responder (n= 48, 5≤ oocyte count≤ 14), and high responder (n= 26, oocyte count≤ 15) patient groups continuing IVF treatment. Results The cumulus cell GST enzyme activity were statistically significantly increased in the high responders group compared to the poor responder and the normo responder's groups (p< 0.001 and p= 0.002, respectively). The cumulus cell MDA levels were significantly decreased in the high responder group compared to the poor responder group (p= 0.008). The cumulus cell CAT (p= 0.175) and ARE (p= 0.124) enzyme activities were examined but no statistically significant difference found between the groups. Conclusion The significant increase in GST enzyme activity and significant decrease in MDA levels in the high responder group indicate that oxidative stress has an effect oocyte status and quality. |
| Anahtar Kelimeler |
| Arylesterase | Catalase | Cumulus cell | Glutathione-S-transferase | In vitro fertilization | Malondialdehyde |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 10 |
| Scopus | 12 |
| Google Scholar | 18 |