Optimization of culture conditions for the production and activity of recombinant xylanase from microalgal platform
Yazarlar (5)
Arş. Gör. Arzu Yıldırım Ege University Faculty Of Engineering, Türkiye
Dr. Öğr. Üyesi Esra İlhan Ayışığı Kırşehir Ahi Evran Üniversitesi, Türkiye
Dr. Öğr. Üyesi Ahmet DÜZEL Sinop Üniversitesi, Türkiye
Stephen Patrick Mayfield
Department Of Molecular Biology, Amerika Birleşik Devletleri
Department Of Molecular Biology, Amerika Birleşik Devletleri
Prof. Dr. Sait Sargın Bursa Teknik Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Biochemical Engineering Journal (Q2) | ||
| Dergi ISSN | 1369-703X Dergi Bilgileri (2023) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | Türkçe | Basım Tarihi | 08-2023 |
| Cilt / Sayı / Sayfa | 197 / 1 / 108967–0 | DOI | 10.1016/j.bej.2023.108967 |
| Makale Linki | http://dx.doi.org/10.1016/j.bej.2023.108967 | ||
| UAK Araştırma Alanları |
Biyoteknoloji
Biyoproses Tasarımı
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| Özet |
| Xylanases are enzymes responsible for the hydrolysis of the heteropolymer xylan. They have wide applications ranging from the bakery, animal husbandry, and textile to pulp and paper industry and biofuel productions. Recombinant xylanase production has been previously reported from different hosts such as bacteria and yeast. Microalgae offer a safe and cost-effective photosynthetic platform for producing recombinant proteins, including therapeutics and industrial enzymes. In this study, we optimized the production of recombinant xylanase expressed and secreted from the green alga, Chlamydomonas reinhardtii. The growth of the culture was optimized using response surface methodology (RSM) based on central composite design (CCD), with two numeric (culture incubation time and agitation rate) and one categoric (light intensity) factors. The optimum biomass concentration was obtained as 0.71 mg/mL … |
| Anahtar Kelimeler |
| Microalgae | Photobioreactor | Recombinant enzyme | Response surface methodology | Xylanase |
Science Direct
Optimization of culture conditions for the production and activity of recombinant xylanase from microalgal platform
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 6 |
| Scopus | 8 |
| Google Scholar | 9 |