Selection of DNA aptamers for the aptamer-assisted magnetic capture of the purified xylanase from Aspergillus niger
Yazarlar (4)
Dr. Öğr. Üyesi Ahmet DÜZEL Sinop Üniversitesi, Türkiye
Öğr. Gör. Burhan Bora Ege Üniversitesi, Türkiye
Prof. Dr. Gaye Öngen Özgen Ege University Faculty Of Engineering, Türkiye
Prof. Dr. Serap Evran Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | International Journal of Biological Macromolecules (Q1) | ||
| Dergi ISSN | 0141-8130 Dergi Bilgileri (2024) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2024 |
| Cilt / Sayı / Sayfa | 257 / 1 / 128540–0 | DOI | 10.1016/j.ijbiomac.2023.128540 |
| Makale Linki | https://www.sciencedirect.com/science/article/pii/S0141813023054399?via%3Dihub | ||
| UAK Araştırma Alanları |
Biyoteknoloji
Biyoproses Tasarımı
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| Özet |
| Xylanases are a group of enzymes that catalyze the hydrolysis of xylan. Xylanases have wide industrial applications, and they can produced by various organisms. In this study, we aimed to develop aptamers for the capture of xylanase produced by a wild-type Aspergillus niger strain. Xylanase was produced by Aspergillus niger in a 5-liter stirred-tank bioreactor and then purified by column chromatography. Magnetic bead-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) was performed to select DNA aptamers specific to the purified xylanase. After nine rounds of selection, next-generation sequencing (NGS) analysis was performed. Four aptamers, namely AXYL-1, AXYL-2, AXYL-3, and AXYL-4, were identified for further characterization. The binding properties of the selected aptamers were characterized by fluorescence quenching (FQ) analysis and an enzyme-linked aptamer assay … |
| Anahtar Kelimeler |
| Aptamer | Protein purification | Xylanase |
Science Direct
Selection of DNA aptamers for the aptamer-assisted magnetic capture of the purified xylanase from Aspergillus niger
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 5 |
| Scopus | 5 |
| Google Scholar | 7 |